The effect of 12 weeks of aerobic-resistance training and Green tea supplementation on the levels of irisin and interleukin6 of overweight middle-aged men

Background: With the advancement of technology in the 21st century and the decline of physical activity, obesity has become widespread. In developing countries, the prevalence of obesity is increasing day by day and people at a younger age suffer from obesity. The World Health Organization estimates that there are over one billion overweight adults in the world (body mass index above 27 (kg / m2). Metabolic syndrome is one of the negative consequences of obesity and is a set of metabolic and vascular risk factors that include hyperglycemia, changes in serum lipids, hypertension, hypertension and abdominal obesity. Central obesity and insulin resistance are two key components of metabolic syndrome that increase with age and unhealthy lifestyle (high calorie diet and inactivity). On the other hand, obesity is associated with mild chronic inflammation. In fact, one of the main causes of obesity-related metabolic disorders has been attributed to chronic inflammation. In the past, adipose tissue was believed to be an ineffective tissue and acted only as a store of triglycerides. But it is now well established that adipose tissue secretes a number of biologically active proteins called adipokines, thereby playing a role in energy homeostasis, systemic inflammation, and the regulation of the body's physiological functions, and possibly in disease. The development of metabolic syndrome is key. Reactive protein C (CRP) is a stable marker of systemic inflammation produced in response to inflammatory intercellular cytokines (IL-6) and tumor necrosis factor alpha (TNF-1) (secreted from adipose tissue) in the liver. In fact, with the onset of middle age, some physiological changes occur in people that affect their health. Changes in this period of life increase some inflammatory factors and decrease some factors affecting health. At the same time, some supplements are used today to improve people's health. The aim of this study was to evaluate the effect of twelve weeks of combined exercise and green tea supplementation on levels of irisin, IL-6 and body composition of overweight middle-aged men. Method: The method of the present study was quasi-experimental with a pretest-posttest design. For this purpose, 40 overweight middle-aged men according to the inclusion criteria such as: middle-aged men with an age range of 40 to 50 years, middle-aged men with a body mass index between 25 to 30 percent, complete health and no disease of people with any From diseases of blood pressure, diabetes, obesity and atherosclerosis, not using supplements (vitamins and minerals, etc.) or medication,  They were divided into three groups: supplement (12), combination training group - placebo (14) and combination training - supplement (14). Samples with age range (year) 47.94 ±2.83, weight (kg) 85.56 ±9.49, height (m) 174.61 ±5.30, body mass index 27/99 ± 2/08(kg / m2) and fat mass 26/02 ± 2/30% . Also, by observing all ethical criteria before and during the research, criteria such as: no participation of the subject more than three sessions during the training course, consumption of other supplements during the training protocol, simultaneous participation in other sports activities such as exclusion criteria Was taken. The combined exercises group performed combined exercises for twelve weeks and 6 sessions per week for 45 to 60 minutes. Aerobic exercises with an intensity of 60 to 75% of maximum heart rate and resistance exercises with an intensity of 60 to 75% were performed with maximum repetition and the control group did not have any exercises during the training period. They did combination exercises. These exercises were performed in this way on consecutive days. The combined supplementation group receives a 500 mg capsule daily one hour before each exercise session. The placebo-combination combination group also receives one 500 mg placebo capsule one hour before each training session. Subjects in the supplement group did no specific exercise and only performed their daily activities and received a 500 mg capsule daily before each meal. Green tea A bag containing the main polyphenols of green tea is completely powdered and then the capsule cover is filled with this powder. For the placebo group, capsules containing cornstarch powder are prepared that will look like green tea extract capsules. Blood samples were taken 24 hours before the start of training and 48 hours after the last training session. One day before the experiment, data on height, weight, fat percentage and body mass index were measured. After familiarizing the subjects with the implementation of the protocol and the correct method of performing the movements, their maximum repetition was calculated according to Barzisenki's formula in the desired movements and based on the principles of training, the principle of increasing the load was observed. Data were analyzed using repeated measures analysis of variance and Bonferroni post hoc test (P≥0.05). Results: The results showed that combined exercise and green tea supplementation caused a significant increase in the amount of irzin (P = 0.001). The results also showed that IL-6 levels decreased significantly after combined training and green tea supplementation (P = 0.001). Overall, the results showed that resistance training with green tea supplementation decreased interleukin 6 and increased irzin after eight weeks in the combined training groups, training + green tea supplementation and green tea supplementation group compared to the control group. These changes were significant in the control group. Result: In general, the results of the present study indicate that performing combined exercises with the consumption of green tea is essential for the health of middle-aged people and improves their physiological condition. These exercises, along with taking antioxidant supplements such as tea, reduce factors such as interleukin-6, which play an important role in initiating inflammatory processes, and increasing important factors such as irzin can lead to aging and obesity, like some diseases, which is recommended. People should use these exercises with the intensities expressed in the present study and take green tea supplements with the recommended dose.